The clinical value of ex vivo expansion of hematopoietic stem cells (HSCs) might be to increase the number of mature cells or the number of primitive stem cells. It is therefore essential to define the aims of hematopoietic cell expansion and adapt the experimental conditions to obtain the expected cell population for successful therapeutic use. HSC transplantation, indeed, requires expansion of all cellular subsets including precursors, progenitors, and primitive stem cells for short- and long-term engraftment of patients.
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